Reagents used in combination with the Strep-tag technology
Need buying or technical advice?
Contact a protein expert!
|
- +49(0)551 50672-0
- Online
- E-mail (please indicate your complete address)
|
Of special interest to Strep-tag® users!!
 IBA-lyse Bacterial Lysis Buffer
|
 |
The new IBA-lyse Bacterial Lysis Buffer is optimized for preparation of a cleared lysate after cytoplasmic expression of Strep-tag fusion proteins.
The buffer is formulated for gentle disruption of E. coli and release of proteins. It consists of a reagent mix compatible with subsequent Strep-tag purification. The protocol provides a simple, rapid, inexpensive and most importantly more reproducible and milder alternative to mechanical methods such as sonication or French Press for preparation of cleared lysates to be submitted to affinity chromatography. In addition, it avoids unwanted heating which occurs with e.g. sonication.
Avidin, which is included in the buffer, blocks biotin contaminations which could damage the Strep-Tactin purification columns or cause background on Western blots.
|
The IBA-lyse buffer comprises:
- Buffer B which contains 1 mM EDTA, Tween 20 and avidin
- Lyophilized lysozyme
- Lysozyme reconstitution buffer
- Lyophilized DNase I
- DNase reconstitution buffer
Order information
see also "how to order"
| product |
amount |
cat. no. |
order & prices |
| IBA-lyse Bacterial Lysis Buffer |
50 ml* |
2-1017-050 |
 |
| IBA-lyse Bacterial Lysis Buffer |
250 ml |
2-1017-250 |
|
*sufficient to treat 2.5 l E. coli culture
Buffer L - Lysis buffer for mammalian cell preparation
Lysis buffer for preparation of cell extracts of mammalian cells.
Order information
see also "how to order"
| product |
amount |
cat. no. |
order & prices |
| Buffer L, Lysis Buffer for mammalian cells) |
100 ml 5x buffer |
2-1020-100 |
|
Desthiobiotin: Reagent for Strep-tag® II Elution
|
 |
Desthiobiotin displaces Strep-tag II proteins at the biotin-binding site of Strep-Tactin® in a competitive manner resulting in a mild elution of the protein. For your convenience, we offer desthiobiotin ready-to-use in solution. If you desire to prepare your own elution buffer suitable for other protocols desthiobiotin is offered as lyophilized powder as well.
Protocols for download
Order information
see also "how to order"
| product |
amount |
cat. no. |
order & prices |
D-Desthiobiotin solution
= 10 x Buffer E* |
25 ml 10 x buffer for 250 ml |
2-1000-025 |
|
| D-Desthiobiotin |
0.107 g for 200 ml 2.5 mM buffer |
2-1000-001 |
|
| D-Desthiobiotin |
1g |
2-1000-002 |
|
| D-Desthiobiotin |
5g |
2-1000-005 |
|
*contains EDTA
Buffer BE - Biotin Elution Buffer
Elution buffer for competitive displacement of purified recombinant Strep-tag® or One-STrEP-tag® fusion proteins from immobilized Strep-Tactin.
This buffer is especially recommended for use with One-STrEP-tag in protein complex or PPI applications since it contains Biotin which has a higher affinity to Strep-Tactin and therefore more efficiently competes with the (One)Strep-tag binding. The regeneration of the Strep-Tactin columns in PPI applications is not recommended to avoid the isolation of cross contaminations. The buffer is also recommended for the elution of MagStrep beads and Strep-Tactin Spin columns.
Please note that after elution with buffer BE the resin cannot be regenerated.
Order information
see also "how to order"
| product |
amount |
cat. no. |
order & prices |
| Buffer BE, Biotin Elution Buffer |
25 ml 10 x buffer |
2-1019-025 |
|
Strep-tag® Regeneration Buffer with HABA
Contains HABA (2-[4'-hydroxy-benzeneazo]benzoic acid) which displaces desthiobiotin at the biotin-binding site regenerating Strep-Tactin. The yellow HABA solution turns red when desthiobiotin has been removed from Strep-Tactin clearly indicating the regeneration process and activity status of the column.
Order information
see also "how to order"
| product |
amount |
cat. no. |
order & prices |
| Strep-tag regeneration buffer with HABA (10 x concentrated) |
100 ml 10 x Buffer R |
2-1002-100 |
|
Strep-tag® Protein Purification Buffer Set
Convenient set for the purification of recombinant Strep-tag fusion proteins on immobilized Strep-Tactin and for the regeneration of the columns.
Order information
see also "how to order"
| product |
amount |
cat. no. |
order & prices |
Strep-tag protein purification buffer set
(10 x concentrated buffers) |
100 ml 10x Buffer W
(W=washing);
25 ml 10x Buffer E
(E=elution);
100 ml 10x Buffer R
(R=regeneration) |
2-1002-001 |
|
Biotin Blocking Buffer
For blocking biotinylated proteins in Western blots, the membrane is incubated with Biotin Blocking Buffer. Use a dilution of 1:1000 in standard Western blot blocking reagent prior to detecting Strep-tag proteins with Strep-Tactin conjugates.
Order information
see also "how to order"
| product |
amount |
cat. no. |
order & prices |
| Biotin Blocking Buffer |
2 ml |
2-0501-002 |
|
Strep-tag® Washing Buffer
For removal of unbound material during Strep-tag protein purification via Strep-Tactin columns
Order information
see also "how to order"
| product |
amount |
cat. no. |
order & prices |
| Strep-tag washing buffer |
100 ml |
2-1003-100 |
|
Anhydrotetracycline
For induction of tet promoter controlled pASK-IBA vectors
Order information
see also "how to order"
| product |
amount |
cat. no. |
order & prices |
| Anhydrotetracycline |
25 mg |
2-0401-002 |
|
| Anhydrotetracycline |
50 mg |
2-0401-001 |
|
Avidin
Prevents biotinylated proteins from binding to Strep-Tactin columns during Strep-tag protein purification
Order information
see also "how to order"
| product |
amount |
cat. no. |
order & prices |
| Avidin |
15 mg |
2-0204-015 |
|
| Avidin |
50 mg |
2-0204-050 |
|
Strep-tag II Peptide
For elution of Strep-tag proteins from StrepMAB-Classic columns
Order information
see also "how to order"
| product |
amount |
cat. no. |
order & prices |
| Strep-tag II Peptide |
1.8 mg |
2-1018-002 |
|
| Strep-tag II Peptide |
5 x 1.8 mg |
2-1018-009 |
|
|
