IBA : Protein Tools

for purification of Strep-tag^® II proteins by gravity flow or under low pressure

Strep-Tactin MacroPrep is suited for all low pressure chromatography applications. The MacroPrep resin exhibits non-specific binding properties differing from those of Sepharose^® or Superflow^® and can, thus, be recommended in cases where suboptimal results are obtained with Sepharose or Superflow and vice versa (see figure below). See also Strep-Tactin Column Evaluation Set.
Since the Strep-tag purification system is designed for column affinity chromatography as opposed to batch purification, we do recommend our pre-packed Strep-Tactin MacroPrep devices for column chromatography.
Our gravity flow columns are available in different sizes with a bed volume of 0.2 ml, 1 ml, 5 ml and 10 ml and can be used without the need for any further special equipment.
Our Strep-Tactin Macroprep cartridges are available as 1 ml and 5 ml H-PR cartridge. They can be connected in series to enlarge capacity. Adapters are described here. The average capacity of the 1 ml Strep-Tactin MacroPrep cartridge is 50-100 nmol recombinant Strep-tag fusion protein per run and the recommended flow rate is 1 ml/minute (equivalent to approximately 20 drops per minute when working with a syringe).

Specifications of Strep-Tactin MacroPrep resin

Binding capacity	1 ml sedimented resin (corresponds to 2 ml of a 50 % suspension) can be used for the one-step purification of 50 to 100 nmol recombinant protein
Support	MacroPrep^® 50 (polymethacrylate)
Bead size	50 µm
Linear flow rate	Up to 300 cm/h can be used for Strep-tag purification
Form (bulk)	50 % suspension in 100 mM Tris/HCl pH 8.0, 1mM EDTA, 150 mM NaCl
Stability	at least 6 months after shipment
Storage	4 °C, DO NOT FREEZE
Shipment	RT

View more information on the Strep-tag purification system.

Protocols for download

For some recombinant proteins, purification characteristics on Strep-Tactin MarcoPrep differ from those on Strep-Tactin Sepharose

The Coomassie stained SDS gel shows the purification of a recombinant protein which exhibits exceptionally high non-specific protein binding. In this case, we have been able to remove the contaminants nearly quantitatively using the Strep-Tactin MacroPrep resin while the contaminants co-eluted with the recombinant protein after the same purification protocol using the Strep-Tactin Sepharose resin. (Please note, that normally behaving recombinant proteins can be purified in a single step to homogeneity using Strep-Tactin Sepharose). This example shows that it may be reasonable to change the resin if non-specific protein binding occurs.

Lane 1, molecular weight standard (kDa);
Lane 2, Strep-Tactin MacroPrep purified protein sample;
Lane 3, Strep-Tactin Sepharose purified protein sample;
Lane 4, soluble raw lysate as applied on each column

Need buying or technical advice?
Contact a protein expert!

+49(0)551 50672-0
Online
E-mail (please indicate your complete address)

Order information
see also "how to order"

product	amount	cat. no.	order & prices
Gravity flow Strep-Tactin MacroPrep mini-columns (0.2 ml bed volume)	5 columns	2-1506-550
Gravity flow Strep-Tactin MacroPrep column (1 ml bed volume)	1 column	2-1506-001
	5 columns	2-1506-050
Gravity flow Strep-Tactin MacroPrep column (5 ml bed volume)	1 column	2-1506-051
Gravity flow Strep-Tactin MacroPrep column (10 ml bed volume)	1 column	2-1506-101
Strep-Tactin MacroPrep cartridge H-PR (1ml bed volume)	1 cartridge	2-1531-001
Strep-Tactin MacroPrep cartridge H-PR (1ml bed volume)	5 cartridges	2-1531-005
Strep-Tactin MacroPrep Cartridge H-PR (5 ml bed volume)	1 cartridge	2-1532-001
Strep-Tactin MacroPrep Cartridge H-PR (5 ml bed volume)	5 cartridges	2-1532-050
Strep-Tactin MacroPrep (50 % suspension)	4 ml	2-1505-002
	20 ml	2-1505-010
	50 ml	2-1505-025
	200 ml	2-1505-100
	1000 ml	2-1505-500

Buffers & reagents



		Strep-Tactin^® MacroPrep^®

Strep-Tactin® MacroPrep®

for purification of Strep-tag® II proteins by gravity flow or under low pressure

Strep-Tactin^® MacroPrep^®

for purification of Strep-tag^® II proteins by gravity flow or under low pressure