Strep-Tactin® Superflow® &
 Strep-Tactin® Superflow® high capacityNew


for purification of Strep-tag® II proteins by gravity flow, low pressure or FPLC
Get more protein with optimal purity!

While Strep-Tactin® Superflow® is now available at very competitive prices the recently launched Strep-Tactin® Superflow® high capacity resin offers improved binding capacity but identical high protein purity compared to standard Strep-Tactin® Superflow®. Thus, it is the perfect solution for Strep-tag® protein purification.


Both Strep-Tactin® Superflow® resins

  • combine outstanding flow characteristics with superior mechanical stability
  • are suitable for increased flow rates and useful for purification of large protein complexes
  • come in pre-packed Strep-Tactin® columns for gravity flow applications
  • or in the new Strep-Tactin® cartridges H-PR* ("highly pressure-resistant") for work with automated workstations
  • yield much higher protein purity (> 95%) than 6xHistidine-tag
    purification on Ni-NTA Superflow®


The new Strep-Tactin® Superflow® high capacity resin

  • provides 2 to 3 times higher binding capacity than standard
    Strep-Tactin® Superflow® (150 – 500 nmol or 3 – 15 mg recombinant Strep-tag® fusion protein per ml sedimented resin)
  • enables a cost effective purification of higher protein amounts with optimal purity

 

Intact VLP purification using Strep-Tactin Superflow: 4.8 MDa VLPs purified!

Figure 1, left to right: M; Rainbow Marker RPN 755, GE Healthcare Biosciences Corp., 1 and 2; different elution fractions of VLPs fused to Strep-tag II after one-step Strep-Tactin affinity purification from a crude lysate of E. coli.
1 liter culture was induced at an OD600 of 0.6 using anhydrotetracycline (AHT) and protein expression was performed at 37°C for 3 hours at 200 rpm. Then, cells were pelleted, resuspended in
20 ml Buffer W (100 mM Tris-Cl, 150 mM NaCl, 1 mM EDTA) and sonicated. The insoluble material was pelleted and the crude lysate was loaded on a Strep-Tactin Superflow column at a flow rate of 1 ml/minute. After washing off the host proteins, the VLPs fused to Strep-tag II were eluted using 2.5 mM desthiobiotin in Buffer W. pASK-IBA7 was used for cloning.


Strep-tag II after purification on Strep-Tactin Superflow, uranyl acetate stain, 40 000x





* Strep-Tactin® Superflow® high capacity cartridges H-PR can directly be applied to
10-32 connections of HPLC and GE Healthcare ÄKTA™ Adapters for other workstations, syringes, peristaltic pumps as well as to connect several cartridges in series to enlarge capacity are listed in the order table above.


Specifications of Strep-Tactin Superflow resin
  Strep-Tactin® Superflow® Strep-Tactin® Superflow® high capacity
Binding capacity 1 ml sedimented resin (corresponding to 2 ml of a 50 % suspension) can be used for the one-step purification of 50 to 100 nmol (up to 3 mg) recombinant protein 1 ml sedimented resin (corresponding to 2 ml of a 50 % suspension) can be used for the one-step purification of 150 to 500 nmol or 3 -15 mg recombinant protein
Support Superflow 6 (6 % agarose, crosslinked) Superflow 6 (6 % agarose, crosslinked)
Bead size 60-160 µm, spherical 60-160 µm, spherical
Linear flow rate 100-300 cm/h is recommended for Strep-tag purification 100-300 cm/h is recommended for Strep-tag purification
Form (bulk) 50 % suspension in 100 mM Tris/HCl pH 8.0,
150 mM NaCl, 1 mM EDTA		 50 % suspension in 100 mM Tris/HCl pH 8.0,
150 mM NaCl, 1 mM EDTA
Stability at least 6 months after shipment at least 6 months after shipment
Storage 4°C, DO NOT FREEZE 4°C, DO NOT FREEZE
Shipment RT RT

 

Special Application

Strep-Tactin Superflow for intact virus like particle (VLP) purification

For the purification of intact VLPs the careful choice of both an ideal affinity tag system and the resin support is important:

1) The affinity chromatography process should be mild in order to keep the VLPs intact which is met by using Strep-tag.

2) The resin support packing within the column must allow penetration of the large VLPs.


While MacroPrep or POROS 20 carriers did not meet criterion (2), since in both cases the viral particles accumulated on top of the column, Sepharose and Superflow allowed the purification. Superflow can be used in FPLC systems whereas Sepharose is only suitable for gravity flow purification. These results, further illustrated in the figures on the left, were kindly provided by L. Stöckl and B. Brandenburg, Robert Koch Institute, Berlin.


For buffers & reagents pease click here
Protocols for download


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Order information
see also "how to order"

product amount Adapters for
H-PR cartridges		 Standard
Strep-Tactin® Superflow® 		Strep-Tactin® Superflow® high capacity
Gravity flow Strep-Tactin® Superflow® column; 1 ml 1 column   2-1207-001 2-1209-001
5 columns   2-1207-050 2-1209-050
Gravity flow Strep-Tactin® Superflow® column; 5 ml 1 column   2-1207-051 2-1209-051
Gravity flow Strep-Tactin® Superflow® column; 10 ml 1 column   2-1207-101 2-1209-101
Gravity flow Strep-Tactin® Superflow® column; 0.2 ml 5 columns   2-1207-550 2-1209-550
Strep-Tactin® Superflow® cartridge H-PR (with 10-32 connection for HPLC and Äkta); 1 ml 1 cartridge   2-1231-001 2-1233-001
5 cartridges   2-1231-005 2-1233-005
Strep-Tactin® Superflow® cartridge H-PR (with 10-32 connection for HPLC and Äkta); 5 ml 1 cartridge   2-1232-001 2-1234-001
5 cartridges   2-1232-005 2-1234-005
Strep-Tactin® Superflow®; 50% suspension 4 ml   2-1206-002 2-1208-002
20 ml   2-1206-010 2-1208-010
50 ml   2-1206-025 2-1208-025
200 ml   2-1206-100 2-1208-100
1000 ml   2-1206-500 2-1208-500
Syringe adapter set (Luer-lock) for H-PR cartridges 3 sets 2-1021-001    
M6 adapter set for H-PR cartridges 1 set 2-1022-001    
1/4-28 adapter set for H-PR cartridges 1 set 2-1023-001    
1/16 inch adapter set for H-PR cartridges 3 sets 2-1025-001    
Coupling adapter set for connecting up to three H-PR cartridges 2 adapters 2-1026-001    

Please note, that the Strep-tag® purification system is designed for column affinity chromatography as opposed to batch purification.



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